CJC-1295 With DAC

Chemical Reference Standard | Laboratory Research Reagent

PubChem CID 91971820 | C165H269N47O46 | MW 3,647.2 g/mol | CAS 446262-90-4

For laboratory research use only. Not for use in humans or animals.

Product Overview

CJC-1295 with DAC is a synthetic growth hormone-releasing hormone (GHRH) analog with a modified GHRH(1-29) backbone and a C-terminal albumin-binding extension. Research topics include peptide identity, GHRH receptor signaling, proteolytic stability, and peptide-albumin bioconjugation in defined laboratory systems.

The DAC modification contains a maleimide group that can react with an accessible thiol, including albumin Cys34, to form a thioether linkage. Binding and conjugation results depend on the protein preparation, matrix, and experimental conditions. Characterize the resulting material rather than assuming complete conjugation.

Chemical Identity and Molecular Architecture

Backbone Substitutions

The modified GHRH(1-29) backbone includes D-alanine at position 2, glutamine at position 8, alanine at position 15, and leucine at position 27. These substitutions are relevant to structure-activity and proteolytic-stability research. The terminal lysine extension in the DAC construct carries the maleimidopropionyl group.

Albumin Bioconjugation Research

Maleimide-thiol chemistry provides a basis for investigating conjugation to albumin Cys34. Laboratory characterization may examine conjugate formation, unbound peptide, stability, and matrix-dependent changes using appropriate analytical methods and controls.

Molecular Structure

GHRH Receptor and Signaling Assays

GHRHR is a Gs-coupled receptor associated with adenylyl cyclase activation and cAMP signaling. Defined cell-based assays can examine receptor-dependent cAMP responses, PKA-associated signaling, CREB-related readouts, and intracellular calcium measurements. Select readouts suitable for the model and distinguish receptor-dependent responses from background or matrix effects.

Record cell identity, receptor expression, assay conditions, controls, and analytical limitations. A biochemical binding result and a cell-based signaling result measure different aspects of the experimental system and require separate interpretation.

Analytical Characterization and Lot Records

Storage and Laboratory Handling

Follow the lot-specific storage information and SDS. Record material condition and storage history. Solubility and stability depend on the solvent system, pH, matrix, and experimental conditions; assess these within the laboratory’s established methods. Use appropriate PPE and institutional chemical-handling and disposal procedures.

Frequently Asked Questions

What is CJC-1295 with DAC and what is its PubChem CID?

CJC-1295 with DAC is a synthetic GHRH analog with a C-terminal albumin-binding modification. Its reference identifiers are PubChem CID 91971820 and CAS 446262-90-4. The molecular formula is C165H269N47O46 and the molecular weight is approximately 3,647.2 g/mol. The construct includes a modified GHRH(1-29) backbone and a maleimidopropionyl-lysine extension.

How is this material classified?

This material is a laboratory reagent for research use only. Not for use in humans or animals. Its catalog description concerns chemical identity, analytical characterization, and laboratory assay context.

What limitations should researchers consider?

Results depend on the experimental model, reagent identity, impurity profile, matrix composition, and assay conditions. Findings from one laboratory system cannot be assumed to apply to another. Include suitable controls and document the methods and limitations of each experiment.

How does the DAC structure differ from the non-DAC structure?

The DAC form includes a C-terminal maleimidopropionyl-lysine extension associated with albumin-binding research. The non-DAC form lacks that extension and has reference identifiers PubChem CID 56841945 and CAS 863288-34-0, with formula C152H252N44O42 and molecular weight approximately 3,367.9 g/mol. Confirm the specified structure when reviewing analytical records.

What laboratory research topics are relevant to CJC-1295 with DAC?

Relevant topics include GHRH receptor binding, cAMP signaling, peptide stability, and albumin bioconjugation in defined biochemical or cell-based assays. Record the experimental matrix, controls, analytical methods, and lot information alongside each result.

What do salt forms and counterions mean for laboratory research?

Peptide preparations may differ in counterion composition, including acetate or trifluoroacetate. Review the lot documentation for the stated form and any available counterion measurements. Counterions, residual solvents, and matrix composition can influence solubility and assay readouts, so assess their relevance to the experimental system.

What laboratory handling information should researchers review?

Consult the SDS and lot-specific storage information before handling the material. Use appropriate laboratory PPE, avoid dust exposure, and follow institutional procedures for chemical handling and disposal. For laboratory research use only. Not for use in humans or animals.

How can a laboratory assess the identity and quality of the DAC material?

Review the COA and lot identifiers, then assess identity using suitable LC-MS and peptide-mapping methods. Interpret the measured mass with the expected ionization, charge states, and mass convention. HPLC can characterize chromatographic purity, while quantitative content, counterion, endotoxin, or trace-metal measurements may be relevant to the assay. Do not infer every analytical result from a single purity percentage.

Chemical Reference Records

PubChem CID 91971820 and CID 56841945 provide chemical reference records for the DAC and non-DAC structures. Review the lot documentation to identify the supplied material form and analytical results.

For laboratory research use only. Not for use in humans or animals.