CJC-1295 without DAC
CJC-1295 Without DAC (Modified GRF 1-29) is a 29-amino-acid synthetic GHRH analogue for laboratory receptor-signaling, cAMP/PKA, peptide-stability, and GH-IGF-1 pathway assays. The listed scaffold has substitutions at positions 2, 8, 15, and 27 and lacks the MPA albumin-binding conjugate. Supplied as lyophilized powder with per-batch COA documentation.
For laboratory research use only. Not for use in humans or animals.
Price range: $46.00 through $65.00
"These Statements Have Not Been Evaluated By The Food And Drug Administration. This Product Is Not Intended To Diagnose, Treat, Cure Or Prevent Any Disease."
CJC-1295 Without DAC
Modified GRF (1-29) | GHRH Analogue Research Reagent
29 Amino Acids | MW 3,367.9 g/mol | PubChem CID 91976842 | Four Stabilising Substitutions | Lyophilized | COA Per Batch | RUO
For laboratory research use only. Not for use in humans or animals.
TECHNICAL SPECIFICATIONS
| Property | Data |
|---|---|
| Product Reference | CJC-1295 Without DAC | Modified GRF (1-29) Research Reagent |
| Also Known As | Mod GRF 1-29 | Modified Growth Releasing Factor (1-29) | GHRH(1-29) Tetrasubstituted Analogue |
| Chemical Class | Synthetic Peptide – GHRH Receptor Agonist Research Reagent |
| PubChem CID | 91976842 |
| Molecular Formula | C152H252N44O42 |
| Molecular Weight | 3,367.9 g/mol (approximate) |
| Sequence Length | 29 amino acids |
| Substitution Sites | Positions 2, 8, 15, 27 – tetrasubstituted DPP-IV resistant scaffold |
| Half-Life (Research Model) | ~30 minutes | Short-acting pulsatile profile |
| Physical Format | Lyophilized powder in a hermetically sealed laboratory container |
| Analytical Purity | ≥99% (RP-HPLC verified) |
| Identity Confirmation | LC-MS – molecular weight and sequence verified per batch |
| Endotoxin Screening | <0.25 EU/mg (LAL method) |
| Laboratory Solvent System | Aqueous laboratory buffer (Solution) |
| Storage – Lyophilized | 2–8°C, desiccated, light-protected |
| Storage – After Solubilization | -20°C, single-use aliquots; protect from repeated freeze-thaw |
| Research Classification | Research Use Only (RUO) |
| COA Availability | Per-batch COA downloadable from Profound Aminos COA portal |
Product Overview
CJC-1295 Without DAC (Modified GRF 1-29) is a tetrasubstituted synthetic analogue of the N-terminal 29-amino-acid fragment of GHRH. GHRH is a 44-amino-acid hypothalamic neuropeptide associated with pituitary somatotroph signaling and the GH-IGF-1 axis. This reagent provides laboratory research context for GHRH receptor (GHRHR) interactions and downstream signaling measurements.
The no-DAC designation identifies the absence of the maleimidopropionic acid (MPA) albumin-binding conjugate. The listed research-model half-life is approximately 30 minutes. Temporal receptor responses, somatostatin interactions, and GH-axis measurements require interpretation within the selected assay system rather than assuming a physiological outcome from material identity alone.
MOLECULAR STRUCTURE
The listed molecular formula is C152H252N44O42 and approximate molecular weight is 3,367.9 g/mol. The existing scaffold description identifies substitutions at positions 2, 8, 15, and 27. Refer to batch sequence documentation for the supplied molecular form.
The listed computed properties include a topological polar surface area of approximately 1,450 Å2, 52 hydrogen-bond donors, 48 acceptors, and 118 rotatable bonds. The existing research description lists an approximate native GHRH half-life of 7 minutes and a Modified GRF model value of 30 minutes; such values depend on molecular form and experimental conditions.
Four Stabilising Amino Acid Substitutions
| Position | Native GHRH Residue | Modified GRF Residue | Research Rationale |
|---|---|---|---|
| 2 | L-Ala | D-Ala | Blocks DPP-IV enzymatic cleavage at the Tyr-Ala N-terminal bond |
| 8 | Gly | Aib / Gln | Prevents Asn deamidation and amide hydrolysis to Asp; enhances metabolic stability |
| 15 | Ala | Ala (modified) | Enhances GHRHR binding bioactivity and receptor engagement kinetics |
| 27 | Met / Ser | Leu | Prevents methionine oxidation during lyophilisation, shipping, and storage |
RESEARCH MECHANISMS AND LABORATORY ASSAYS
GHRHR-cAMP-PKA Signaling
Research contexts include GHRHR binding kinetics, adenylate-cyclase signaling, intracellular cAMP measurements, PKA substrate phosphorylation, GH gene transcription, and secretory-vesicle readouts. The existing description lists GH3 and HP75 cell-line contexts; verify receptor expression and suitability for the particular assay.
Temporal Signaling and Somatostatin Interactions
Controlled assays can examine the timing of GHRH-related signaling alongside somatostatin (SRIF) inhibitory pathways. Relevant endpoints include temporal GH measurements, SRIF pathway interactions, and feedback-response kinetics under defined experimental conditions.
GH-IGF-1 Pathway Measurements
Laboratory research contexts include JAK2-STAT5 signaling, IGF-1 expression, IGF-1 ELISA measurements, IGFBP-3 regulation, and GH-receptor response kinetics in hepatocyte culture systems. Interpret each endpoint according to the specific model and analytical controls.
DPP-IV Resistance and Peptide Stability
Biochemical assays examine the contribution of the listed substitutions to enzymatic degradation, including DPP-IV cleavage and residue-associated oxidation or hydrolysis. DPP-IV degradation assays, matrix stability measurements, and time-course analyses provide context for evaluating the supplied peptide scaffold.
GHRHR and GHS-R1a Pathway Interactions
Receptor-pathway research may examine GHRHR-associated cAMP/PKA signaling and GHS-R1a-associated phospholipase-C/calcium signaling in controlled cellular assays. Relevant measurements include calcium mobilization, receptor responses, and multi-component assay interactions. Combined effects must be evaluated within the selected experimental system.
Model-Dependent Somatotroph Responses
Somatotroph secretory capacity and hypothalamic-pituitary feedback sensitivity are model-dependent research endpoints. Time-course and model-stratified measurements require suitable controls and do not establish a general age-related outcome.
GHRH SCAFFOLD RESEARCH CONTEXT
The following table retains the existing scaffold descriptors and research-model values. It provides molecular and temporal assay context. Values depend on molecular form and experimental conditions.
| Feature | CJC-1295 Without DAC (Mod GRF 1-29) | CJC-1295 With DAC | Sermorelin (GRF 1-29) | Native GHRH (1-44) |
|---|---|---|---|---|
| Amino Acids | 29 | 29 + DAC conjugate | 29 | 44 |
| Substitutions | 4 (DPP-IV resistant) | 4 + MPA linker | 0 (native 1-29) | 0 (native) |
| Half-Life | ~30 min | ~6-8 days | ~7-11 min | ~7 min |
| GH Profile | Pulsatile – discrete | Tonic – sustained | Pulsatile – rapid | Pulsatile – rapid |
| DPP-IV Resistance | High | High | Low | None |
| Albumin Binding | No | Yes (MPA-mediated) | No | No |
| Temporal Assay Context | Discrete pulse measurements | Sustained signaling measurements | Rapid washout | Rapid washout |
| PubChem CID | 91976842 | N/A (conjugate) | Multiple | N/A |
WHY SOURCE FROM PROFOUND AMINOS
Sequence and Analytical Identity Documentation
RP-HPLC characterizes analytical purity, while LC-MS and sequence profiling provide identity information. Profound Aminos lists both RP-HPLC purity verification and LC-MS identity confirmation per batch. Review the documentation for the specific substitution pattern and material identity.
Lyophilized Laboratory Format
Lyophilization removes aqueous solvent to provide a dry peptide material. Stability and assay concentration accuracy depend on material characterization, solvent compatibility, storage conditions, and analytical measurements after solubilization.
USA Domestic Cold-Chain Logistics
Profound Aminos lists USA domestic shipping through monitored cold-chain materials handling from a climate-controlled facility. Temperature management is relevant to evaluating peptide aggregation, oxidation, and deamidation during storage and transport.
Endotoxin Screening
Endotoxin contamination can confound cell-based signaling assays. The listed per-batch screening limit is below 0.25 EU/mg by the LAL chromogenic method, with results documented on the COA.
RESEARCH USE AND LABORATORY HANDLING
For laboratory research use only. Not for use in humans or animals.
Use appropriate gloves, a lab coat, and protective eyewear during solubilization and handling. Follow institutional safety procedures and consult the applicable material safety documentation.
Store lyophilized material at 2–8°C, desiccated and protected from light. After solubilization, store at −20°C in single-use aliquots and limit repeated freezing and thawing. The listed solvent system is an aqueous laboratory buffer (Solution); assess compatibility with the specific assay.
Frequently Asked Questions
What is the intended use of CJC-1295 Without DAC?
For laboratory research use only. Not for use in humans or animals.
What does the no-DAC designation describe?
The no-DAC material lacks the maleimidopropionic acid (MPA) albumin-binding conjugate described for the DAC-modified form. The listed research-model half-life is approximately 30 minutes and depends on the experimental system.
What does Modified GRF (1-29) mean?
The name describes a 29-amino-acid GHRH fragment with substitutions at positions 2, 8, 15, and 27. The existing material specification lists PubChem CID 91976842, formula C152H252N44O42, and approximate molecular weight 3,367.9 g/mol.
What solvent system is listed for laboratory solubilization?
The listed solvent system is an aqueous laboratory buffer (Solution). Select assay concentrations and buffer conditions according to the specific method and material documentation. Store solubilized material at −20°C in single-use laboratory aliquots and limit repeated freezing and thawing.
What research classification applies to this material?
This material is supplied as a Research Use Only laboratory reagent for analytical characterization and controlled biochemical or cellular assays.
How should researchers interpret related GHRH analogue literature?
Identify the exact molecular form, experimental model, and assay conditions in each publication. Findings for albumin-conjugated materials or other GHRH scaffolds do not establish identical properties for the supplied no-DAC material.
Do you provide a COA for every batch?
Yes. Each batch includes a Certificate of Analysis documenting RP-HPLC purity of ≥99%, LC-MS identity confirmation, endotoxin screening below 0.25 EU/mg by the LAL method, lot number, and manufacturing date. The COA is available through the Profound Aminos COA portal.
What laboratory handling precautions apply?
Use suitable laboratory gloves, a lab coat, and protective eyewear during solubilization and handling. Follow institutional safety procedures and consult the applicable material safety documentation.
KEY REFERENCE IDENTIFIERS
The existing bibliography identifies Ionescu and Frohman (2006), PMID 16682504; Bowers et al., PMID 10372741; Alba et al. (2006), doi:10.1152/ajpendo.00201.2006; Raun et al. (1998), PMID 9849822; and PubChem CID 91976842 at pubchem.ncbi.nlm.nih.gov/compound/91976842. Consult the original records for the exact molecular forms, experimental systems, and endpoints studied. Related scaffold literature does not establish the properties of every supplied material.
For laboratory research use only. Not for use in humans or animals.
| Weight | N/A |
|---|---|
| Dimensions | N/A |
| Strength | 10mg, 5mg |
DISCLAIMER:
- Products sold on our website are meant for scientific research purposes only, designed for in vitro testing and lab experimentation exclusively. These products are not intended to be used as foods, drugs or cosmetics, any sort of bodily introduction of the products into humans or animals is strictly prohibited. They must also not be misbranded, misused, or mislabeled, or used for anything other than research and scientific investigation.
- All the products you see on the website are being sold in a lyophilized powder state (freeze-dried), in a sealed sterile vial; and should be reconstituted.
The product’s label clearly states the amount of product a vial contains; some products are offered in different variations. - The products we are selling come in a sealed vial but require additional lab equipment for proper testing.
- Though we make sure packaging, label, seals and writing does not differ from the product photos you see on our website, there is a chance for a minimal deviation.
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