Kisspeptin-10 (KP-10) | Research Reagent

TECHNICAL SPECIFICATIONS

• Product Reference: Kisspeptin-10 (KP-10) Research Reagent
• Chemical Class: Synthetic decapeptide, KISS1 gene-product fragment
• Physical Format: Lyophilized powder in a hermetically sealed laboratory container
• Net Content: Per container; consult the product listing and batch COA
• CAS Registry: 374675-21-5
• PubChem CID: 25240297
• Molecular Formula: C₆₃H₈₃N₁₇O₁₄
• Molecular Weight: 1302.4 Da
• Sequence: Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH₂ (YNWNSFGLRF-NH₂)
• Analytical Purity: ≥99% by RP-HPLC
• Identity: LC-MS mass and sequence confirmation
• C-terminal Modification: Verified amidation, Phe-NH₂
• Salt Form: TFA salt; net peptide content stated on COA
• Endotoxin Screening: <0.25 EU by LAL assay; consult batch COA
• Listed Solvent Systems: Aqueous buffer, laboratory saline, or dilute 1% acetic acid, subject to assay compatibility
• Lyophilized Storage: 2–8°C, desiccated and protected from light
• Solution Storage: −20°C, single-use laboratory aliquots

For laboratory research use only. Not for use in humans or animals.

Product Description

Kisspeptin-10 is a synthetic decapeptide corresponding to the conserved C-terminal fragment of the KISS1 gene product. The listed 145-amino-acid precursor gives rise to KP-54, KP-14, KP-13, and KP-10, which share the RF-amide terminus. KP-10 provides a defined molecular material for in-vitro KISS1R receptor and signaling assays.

KISS1R (GPR54) is described as a class A GPCR with Gq/11-associated signaling. The listed KISS1 gene locus is chromosome 1q32.1 and the receptor locus is chromosome 19p13.3. C-terminal amidation, salt form, and net peptide content are relevant identity parameters for interpretation of laboratory measurements.

Research Mechanisms and Laboratory Applications

KISS1R Binding and Gq Signaling

Assay contexts include phospholipase C (PLC), IP3/DAG signaling, intracellular Ca²⁺ measurements, PKC activation, ERK1/2 phosphorylation, and β-arrestin recruitment. The existing binding description lists Ki approximately 2.33 nM for human KISS1R and EC50 approximately 1.0 nM in a CHO-GPR54 IP accumulation assay (PMC5790198). Interpret these values according to the specific assay conditions.

Research endpoints include IP1 accumulation by HTRF, fluorometric calcium measurements, receptor binding, and phosphorylation panels. PDB 8XGS provides structural research context for docking and molecular-contact analysis.

Neuroendocrine Pathway Measurements

Laboratory research contexts include GnRH-associated signaling, LH/FSH secretion kinetics in ex-vivo systems, and steroidogenesis markers in cell cultures. CHO and HEK293 receptor-expression systems provide additional contexts for controlled signaling assays.

Cell Migration and Receptor Expression

KISS1-associated research includes cell motility, proliferation, MAPK signaling, radioligand binding, and KISS1R expression measurements. Findings from modified probes or conjugates require interpretation according to their exact molecular identity and experimental model.

Metabolic Signaling Interfaces

Laboratory endpoints include pancreatic-islet morphology, hormone secretion panels, enteroendocrine cell markers, and GLP-1/GIP-associated measurements. These provide assay contexts for studying relationships between kisspeptin and metabolic signaling.

Endothelial and Hepatic Assays

Research contexts include VEGF ELISA, endothelial migration assays, HUVEC proliferation measurements, and hepatic extracellular-matrix markers. Interpret each readout within the defined cell model, concentrations, and controls.

Granulosa-Cell Signaling

The existing description lists let-7b/ITGB7, ERK, PI3K, and Akt pathway research. Laboratory endpoints include progesterone quantification, phosphorylation measurements, flow-cytometric cell-state analysis, and EdU proliferation assays.

Structural and Identity Parameters

MOLECULAR STRUCTURE

The retained images provide molecular structure references for Kisspeptin-10. Confirm material identity and C-terminal amidation using the batch analytical documentation.

Isoform Research Context

The existing description lists TFA salt forms and variable registry assignments for longer isoforms. Confirm the precise molecular form and net peptide content for each material. Shared sequence features do not establish identical assay behavior.

WHY SOURCE FROM PROFOUND AMINOS

C-terminal Amidation Documentation

Profound Aminos lists LC-MS fragmentation analysis for verification of Phe-NH₂ amidation. Amidated and free-acid forms are distinct identity parameters relevant to receptor assays. Review both purity and structural documentation for the supplied lot.

Net Peptide Content

The batch COA states net peptide content separately from gross salt weight. This information supports calculation of peptide concentrations in analytical assays. The existing bibliography identifies PMC4830481 for peptide-characterization context.

USA Cold-Chain Materials Handling

Profound Aminos lists USA domestic dispatch from a climate-controlled facility using monitored cold-chain handling. Tryptophan and tyrosine residues provide context for evaluating light-associated and oxidative modifications. Review storage conditions alongside lot documentation.

RESEARCH USE AND LABORATORY HANDLING

For laboratory research use only. Not for use in humans or animals.

Wear suitable gloves, a lab coat, and protective eyewear during solubilization and handling. Follow institutional safety procedures and consult the SDS for CAS 374675-21-5.

Store lyophilized material at 2–8°C, desiccated and protected from light. Store solutions at −20°C in single-use aliquots and limit repeated freezing and thawing. Assess compatibility and stability in the selected aqueous buffer, laboratory saline, or dilute acetic-acid solvent system.

Frequently Asked Questions

What is the intended use of Kisspeptin-10?

For laboratory research use only. Not for use in humans or animals.

What structural relationship links the kisspeptin isoforms?

KP-54, KP-14, KP-13, and KP-10 are described as cleavage products of the KISS1 precursor. They share the conserved C-terminal decapeptide sequence and RF-amide terminus. KP-10 contains ten amino acids.

Why is C-terminal amidation relevant to KISS1R assays?

The listed KP-10 sequence ends in Phe-NH₂. Amidation status is an identity parameter relevant to receptor-binding measurements. Review the batch LC-MS fragmentation data and COA for the supplied form.

What analytical data is included with each batch?

The listed COA includes RP-HPLC purity of ≥99%, LC-MS mass and sequence confirmation, C-terminal amidation verification, salt form, net peptide content, endotoxin screening below 0.25 EU by the LAL assay, lot number, and manufacturing date.

Which cell systems provide KISS1R assay context?

The existing research description lists CHO-GPR54 cells for IP accumulation, HEK293 cells expressing KISS1R for calcium and ERK measurements, and GT1-7 neuronal cells for GnRH-associated pathway work. Verify receptor expression and assay suitability for the specific experimental system.

What research classification applies to this material?

Kisspeptin-10 is supplied as a Research Use Only laboratory reagent for receptor-binding, cellular signaling, and analytical assays.

What storage and handling conditions are listed?

Store lyophilized material at 2–8°C, desiccated and protected from light. Store laboratory solutions at −20°C in single-use aliquots. Use gloves, a lab coat, and protective eyewear, and consult the SDS for CAS 374675-21-5.

What structural data provides research context for KP-10?

The existing bibliography identifies PDB 8XGS and PMC11328905 for KISS1R structural research. Consult the original records for molecular contacts, receptor coupling, and experimental conditions.

How should related literature be interpreted?

Confirm the exact peptide form, salt, receptor system, concentrations, and controls in each publication. Findings for modified conjugates or other kisspeptin isoforms do not establish identical properties for the supplied KP-10 material.

What metabolic signaling endpoints are described for laboratory research?

Research contexts include pancreatic-islet morphology, hormone secretion panels, enteroendocrine cell markers, and GLP-1/GIP-associated signaling measurements. Interpret these endpoints within the defined assay model and analytical controls.

Existing Scientific Reference Identifiers

The existing bibliography identifies PMC11328905; PMC5790198; PMC12112028; PMC4830481; doi:10.1021/acs.molpharmaceut.5c01853; doi:10.64898/2026.02.04.703727; Biomolecules 2025;15(11):1591; Int J Mol Sci 2025;26(10):4890; Domest Anim Endocrinol 2025;92:106947; Oakley AE et al., Endocr Rev 2009;30(6):713; Kotani M et al., J Biol Chem 2001;276(37):34631; NCBI Gene KISS1 (3814); and KISS1R (84634). Consult the original records for compound identity, experimental conditions, and endpoints studied.

For laboratory research use only. Not for use in humans or animals.