ARA-290 (Cibinetide) 10 mg | Laboratory Research Peptide

EPOR/CD131 Receptor-Signaling Research | 11-Amino Acid Peptide | Lyophilized Format

For laboratory research use only. Not for use in humans or animals.

TECHNICAL SPECIFICATIONS

CHEMICAL IDENTITY AND STRUCTURAL CONTEXT

ARA-290, also known as cibinetide or pHBSP, is an 11-residue synthetic peptide based on the helix-B surface domain of erythropoietin. Its sequence is pGlu-Glu-Gln-Leu-Glu-Arg-Ala-Leu-Asn-Ser-Ser, or ZEQLERALNSS with Z representing pyroglutamate. The listed EPO surface-domain context includes residues 11–15 and 100–108 in tertiary proximity.

The molecular formula stated in the chemical-identity section is C51H84N16O21 and the molecular weight is 1,257.3 g/mol. Registry references are CAS 1208243-50-8, PubChem CID 91810664, ChEMBL3545305, KEGG D11218, UNII 9W5677JKDA, and DB13006.

N-terminal pyroglutamate results from glutamine cyclisation and is associated with resistance to aminopeptidase degradation. Arginine at position 6, corresponding to EPO Arg-103, is relevant to EPOR/CD131 receptor engagement and hydrogen-bonding interactions.

Listed computed properties include 13 hydrogen-bond donors, 23 acceptors, and a topological polar surface area of approximately 528 Ų. The formula contains 16 nitrogen atoms, consistent with the peptide’s amino-acid composition.

MOLECULAR STRUCTURE AND PATHWAY SCHEMATIC

ARA-290 sequence and EPOR/CD131 pathway schematic, PubChem CID 91810664, CAS 1208243-50-8. The image provides structural and receptor-signaling context.

RECEPTOR AND CELL-BASED ASSAY CONTEXT

The innate repair receptor (IRR) is described as the EPOR/CD131 heterodimer, with CD131 also known as CSF2RB or the beta-common receptor. ARA-290 is listed as a selective IRR agonist with a non-erythropoietic research profile. This receptor context is distinct from the EPOR homodimer.

1. Receptor-binding measurements: Binding-affinity quantification, competitive displacement, and receptor-saturation measurements in EPOR/CD131-expressing cell systems.
2. NF-kB and cytokine measurements: NF-kB p65 nuclear localization and DNA binding by EMSA, cytokine ELISA panels for IL-6, TNF-α, and IL-12, NOS2 activity, CXCL-10 readouts, and flow-cytometric myeloid-cell markers in LPS-stimulated culture systems.
3. JAK2/STAT5 and MAPK measurements: Western blot analysis of pJAK2, pSTAT5, and pERK1/2, EPOR/CD131 co-immunoprecipitation, kinase-inhibitor competition, and STAT5 reporter assays.
4. Cellular readouts: TRPV1 calcium imaging and patch-clamp recordings, caspase-3/7 activity, TUNEL staining, JC-1 membrane-potential measurements, ATP bioluminescence, and transwell permeability assays.
5. Additional assay endpoints: Myeloperoxidase activity, multiplex cytokine panels, LC3B-II/LC3B-I autophagy measurements, mitochondrial permeability-transition assays, insulin-secretion ELISA, and IFN-γ ELISpot measurements are discussed in the referenced literature.

These endpoints describe laboratory methods and pathway questions. Experimental findings must be interpreted in the context of the specific cell system, assay conditions, and analytical controls.

ANALYTICAL DOCUMENTATION AND MATERIAL HANDLING

Each batch is documented by RP-HPLC purity of ≥99%, LC-MS molecular-weight confirmation and sequence mapping, and LAL chromogenic endotoxin screening below 0.25 EU/mg. The expected protonated molecular ion is [M+H]+ 1258.3. The COA includes the lot number, manufacturing date, storage conditions, and analytical results.

Endotoxin control is relevant because LPS activates NF-kB through TLR4 and can confound concentration-response measurements in IRR-signaling assays.

Store the lyophilized 10 mg material at 2–8 °C, desiccated and protected from light. Asparagine at position 9 is susceptible to deamidation in aqueous solution, including isoaspartate formation that can affect receptor-binding measurements.

PBS or another aqueous diluent provides the listed laboratory solvent context. Evaluate solvent compatibility and solution stability for the selected assay. Store laboratory solution aliquots at −20 °C and limit repeated freezing and thawing. Follow the batch documentation.

Use gloves, a laboratory coat, and protective eyewear during laboratory handling and solubilization. Profound Aminos lists domestic USA shipping via monitored cold-chain logistics from a climate-controlled facility.

SCIENTIFIC REFERENCES

PubChem CID 91810664 provides the compound identity reference. Scientific Reports (2017), DOI 10.1038/s41598-017-13046-3, provides CD131/JAK2 and NF-kB research context. The cited Watanabe et al. and Yao et al. studies provide islet-cell ATP and caspase assay context. Aging (2023), PMC9889362, provides autophagy and cytokine research context.

FREQUENTLY ASKED QUESTIONS

What is the intended research context for ARA-290?

ARA-290 is supplied as a laboratory reagent for receptor-signaling, analytical, and cell-based research assays. For laboratory research use only. Not for use in humans or animals.

What receptor complex is referenced for ARA-290 research?

The innate repair receptor (IRR) is described as a heterodimer of EPOR and CD131, also known as CSF2RB or the beta-common receptor. Research examines receptor binding and downstream JAK2/STAT5 and MAPK signaling in non-haematopoietic cell contexts.

How is ARA-290 structurally related to erythropoietin?

ARA-290 is an 11-residue synthetic peptide based on the helix-B surface domain of EPO. Full-length EPO is a 166-amino acid glycoprotein. The listed ARA-290 receptor profile is selective for EPOR/CD131, with a non-erythropoietic research profile.

What chemical identifiers are listed for ARA-290?

ARA-290, also known as cibinetide or pHBSP, is listed under CAS 1208243-50-8, PubChem CID 91810664, ChEMBL3545305, KEGG D11218, and UNII 9W5677JKDA. The molecular formula is C51H84N16O21 and molecular weight is 1,257.3 g/mol. Its sequence is ZEQLERALNSS, with Z representing pyroglutamate.

What solvent-system considerations apply to laboratory assays?

PBS or another aqueous diluent is listed for laboratory solubilization. Select the solvent system and concentration according to assay requirements and material documentation. The molecular weight of 1,257.3 g/mol provides the basis for molar-concentration calculations.

What storage conditions are listed?

Store the lyophilized material at 2–8 °C, desiccated and protected from light. Laboratory solutions are listed for −20 °C storage in aliquots intended for one analytical use. Limit repeated freezing and thawing and follow the lot-specific documentation.

Which analytical endpoints are described in the component literature?

Listed endpoints include NF-kB DNA binding by EMSA, cytokine ELISA panels, phosphoprotein measurements for JAK2, STAT5, and ERK1/2, receptor-complex co-immunoprecipitation, and transcriptional reporter assays. These provide laboratory assay context and do not establish outcomes for the supplied reagent.

Why is endotoxin screening relevant to these assays?

LPS can activate NF-kB through TLR4 and confound signaling measurements. The listed batch screening specification is less than 0.25 EU/mg using the LAL chromogenic method, with results documented on the COA.

What does the batch COA document?

Each batch COA lists RP-HPLC purity of at least 99%, LC-MS molecular-weight and sequence-identity data, endotoxin screening, lot number, manufacturing date, and storage conditions. Batch COAs are available through the Profound Aminos COA portal, with third-party analytical verification available on request.

Which sequence features are relevant to material handling?

The N-terminal pyroglutamate provides structural context for resistance to aminopeptidase degradation. Arginine at position 6 is associated with receptor engagement. Asparagine at position 9 is susceptible to deamidation in aqueous solution, which can affect receptor-binding measurements.

For laboratory research use only. Not for use in humans or animals.