5-Amino-1MQ | Small-Molecule NNMT Laboratory Reagent

Technical Specifications

• Class: Quinolinium small molecule, NAM-competitive NNMT inhibitor
• Target: Nicotinamide N-methyltransferase, EC 2.1.1.1
• Cation name: 1-methylquinolin-1-ium-5-amine
• Iodide CAS: 42464-96-0
• PubChem: Cation 950107; iodide 66522933
• Formula: Cation C10H11N2+; iodide C10H11IN2
• Mass: Cation 159.21 g/mol; iodide 286.11 g/mol
• SMILES: C[n+]1cccc2c(N)cccc12
• InChIKey: ZMJBCEIHNOWCMC-UHFFFAOYSA-O
• Format: Encapsulated laboratory aliquots, listed 50 mg or 100 mg nominal configurations; confirm batch content
• Container: Sealed amber laboratory bottle with desiccant and tamper-evident seal
• Purity: Listed ≥98%, RP-HPLC
• Identity: LC-MS; 1H-NMR for reference batches
• Solvents: Water and DMSO; verify buffer compatibility
• Endotoxin: When specified, <1.0 EU/mg by LAL assay

For laboratory research use only. Not for use in humans or animals.

Product Overview

5-Amino-1MQ is a quinolinium small-molecule reagent for NNMT assays. NNMT transfers a methyl group from SAM to nicotinamide (NAM), producing 1-methylnicotinamide (1-MNA). This reaction provides context for methyl-transfer biochemistry, NAM handling, and NAD+-associated metabolic measurements.

The N-methylated quinolinium core and 5-amino substitution provide structural contexts for substrate-pocket interactions. Cellular permeability and target engagement require measurement in the selected experimental system.

Biochemical and Cellular Assays

The existing specification lists biochemical IC50 ~1.2 µM with recombinant human NNMT at 50 µM SAM and 100 µM NAM. Listed cellular values include ~2.3 µM for 1-MNA reduction and ~30 µM for a lipogenic endpoint in 3T3-L1 systems. Interpret values within the methods and concentrations actually studied.

• NNMT enzyme activity, NAM competition, and methyltransferase counter-screens
• 1-MNA quantification by LC-MS and concentration-response curves
• NAD+, NAD+/NADH, SAM:SAH, NAMPT, and NMNAT measurements
• 3T3-L1 cellular OCR and ECAR assays; SREBP-1c, FAS, ACC, and DGAT panels
• C2C12 cell-state measurements, 5-EdU incorporation, myogenin and MyHC markers, proteomics and metabolomics
• PAMPA, Caco-2 permeability, and intracellular concentration measurements

Structural Characterization

Quinolinium charge, amino substitution position, and counterion identity inform material assessment. RP-HPLC purity alone does not establish substitution position or salt composition. Orthogonal characterization may include LC-MS/MS, 1H-NMR aromatic assignments, elemental analysis, and residual-solvent testing.

Salt Form and Concentration Calculations

The page lists 1.89 mg iodide salt as equivalent to 1.0 mg cation, alongside masses of 286.11 and 159.21 g/mol. This conversion requires reconciliation against molecular masses and actual batch composition before use in calculations. Confirm salt form, net content, and water content using the batch record.

Alternative counterions have different molecular masses and solvent behavior. Review iodide or chloride analytical information, elemental results, and actual material specifications rather than relying on nominal capsule mass.

Research Material Context

Related assay materials include endogenous 1-MNA/MNAM (listed CAS 3106-60-3), tricyclic NNMT inhibitor scaffolds, and bisubstrate SAM-NAM conjugates. These provide distinct molecular and binding contexts; their responses depend on material identity and assay design.

Batch Documentation

Listed documentation includes RP-HPLC purity, LC-MS identity, salt confirmation, lot number, storage conditions, residual-solvent screening, and applicable endotoxin results. For encapsulated materials, carrier and shell composition are relevant to assay compatibility. Synthesis provenance, intermediate characterization, and elemental or heavy-metal results provide additional material-assessment context.

Storage and Laboratory Handling

Keep solid material dry, dark, and desiccated. The existing storage description permits room temperature and recommends −20°C for longer-term material retention. Dissolved stocks are listed at −80°C in single-use aliquots. Limit repeated freezing and thawing.

Use nitrile gloves, a lab coat, and protective eyewear. Consult the batch SDS before handling. Evaluate solvent compatibility and solution stability within the intended laboratory method.

Existing Scientific Reference Identifiers

The existing bibliography lists Neelakantan et al., doi:10.1016/j.bcp.2017.11.007 and doi:10.1016/j.bcp.2019.02.008; Dimet-Wiley et al., PMID 38969654, doi:10.1038/s41598-024-66034-9; Sun et al., PMID 38919254; Kraus et al. (2014); Swaminathan et al. (2017); Ruf et al. (2022); Zhang et al. (2025); Sigma SML2832; and PubChem CIDs 950107 and 66522933. Consult original records for exact materials and experimental endpoints.

For laboratory research use only. Not for use in humans or animals.